abstract：:Mast cells are not only the primary effector cells of immediate type immune reactions, but they have recently also been considered to contribute to the induction of an immune response. Data on the ability of the cells to express major histocompatibility complex (MHC) antigens and the mechanisms involved are however co...

journal_title：Journal of dermatological science

authors： Grabbe J,Karau L,Welker P,Ziegler A,Henz BM

更新日期：1997-11-01 00:00:00

abstract：:The influence of dibutyril cyclic AMP (dbcAMP) on the gene expression of matrix metalloproteinases (MMP)-1, MMP-2, MMP-3, tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 was investigated in human skin fibroblasts by northern analyses. The treatment of dbcAMP increased MMP-1 and MMP-3 mRNA levels but decrea...

journal_title：Journal of dermatological science

authors： Kuroda K,Shinkai H

更新日期：1997-08-01 00:00:00

abstract：:Hamster flank organs are useful for studying androgen-dependent growth of hair follicles and sebaceous glands. A cDNA clone (FAR-17a) was isolated from the hamster flank organ, whose expression was highly sensitive to androgen. The mRNA level of this gene was reduced after castration but reappeared after testosterone ...

journal_title：Journal of dermatological science

authors： Aoki H,Seki T,Sawada J,Handa H,Adachi K

更新日期：1997-05-01 00:00:00

abstract：:Examining damage (inactivation) of antioxidant enzymes in the cells and the pattern of recovery after a single UV exposure might be a useful method for analyzing the mechanisms of chronic UV irradiation, because chronic UV irradiation means repeated single exposures. We irradiated human skin fibroblasts with a single ...

journal_title：Journal of dermatological science

authors： Shindo Y,Hashimoto T

更新日期：1997-03-01 00:00:00

abstract：:We have examined the effects of four 'exogenous' growth factors, i.e. PDGF-BB (5 ng/ml), TGF-beta1 (5 ng/ml), bFGF (10 ng/ml) and EGF (10 ng/ml) on the contraction of floating collagen type I lattices populated by human normal skin (NS) and hypertrophic scar (HS) fibroblasts (FPCL). Only TGF-beta1 enhanced the contrac...

journal_title：Journal of dermatological science

authors： Yang CC,Lin SD,Yu HS

更新日期：1997-02-01 00:00:00

abstract：:Skin biopsies from four patients with similar clinical phenotypes of non-lethal junctional epidermolysis bullosa (JEB) were investigated by electron microscopy and immunohistochemistry. Electron microscopy revealed cleavage within the lamina lucida in each case, but in addition, there was considerable variability in t...

journal_title：Journal of dermatological science

authors： McGrath JA,Eady RA

更新日期：1997-01-01 00:00:00

abstract：:It has recently been demonstrated that genetic defects in keratin genes cause a number of different skin disorders, including epidermolysis bullosa simplex (EBS), epidermolytic hyperkeratosis (EH), the EH form of epidermal nevi, epidermolytic and non-epidermolytic forms of palmoplantar keratoderma (EPPK and PPK) and p...

journal_title：Journal of dermatological science

authors： Fuchs E

更新日期：1996-12-01 00:00:00

abstract：:Herpes gestationis (HG) is a rare pregnancy-associated disease. The aim of this study was to compare various immunohistochemical and immunobiochemical techniques with respect to their diagnostic sensitivity for HG. We studied 43 HG sera; only half of these reacted with the basement membrane zone (BMZ) with both indire...

journal_title：Journal of dermatological science

authors： Murakami H,Amagai M,Higashiyama M,Hashimoto K,Chorzelski TP,Bhogal BS,Jenkins RE,Black MM,Zillikens D,Nishikawa T,Hashimoto T

更新日期：1996-11-01 00:00:00

abstract：:We have previously reported that two species of pro alpha 1(IV) collagen chains, disulfided (500 kDa) and non-disulfided (180 kDa), were produced by B16 melanoma cells (J Biochem (1994) 116; 1039-1043). The mechanism by which the non-disulfided pro alpha 1(IV) chain is produced was studied. No significant difference i...

journal_title：Journal of dermatological science

authors： Tajima S,Tokimitsu I

更新日期：1996-10-01 00:00:00

abstract：:FK506, a new immunosuppressive agent, is effective in treating patients with psoriasis. A major feature of psoriasis vulgaris is the hyperproliferation of keratinocytes together with inflammation. To determine the effect of FK506 or cyclosporin A (CsA) on the keratinocyte cell cycle, flow cytometry and the growth fact...

journal_title：Journal of dermatological science

authors： Karashima T,Hachisuka H,Sasai Y

更新日期：1996-09-01 00:00:00

abstract：:p53, A tumor suppressor gene, has been documented as the most frequently mutated gene in human cancers including non-melanoma skin tumors. It has been controversial whether the p53 gene mutation plays a major role for melanoma genesis. To examine the role of p53 in human malignant melanoma carcinogenesis, we performed...

journal_title：Journal of dermatological science

authors： Kanoko M,Ueda M,Nagano T,Ichihashi M

更新日期：1996-06-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) has been demonstrated to selectively decrease the production of type I and type III collagens in human dermal fibroblasts. The effects of the commonly used food antioxidants, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, propyl gallate, supero...

journal_title：Journal of dermatological science

authors： Taniguchi S,Furukawa M,Kono T,Hisa T,Ishii M,Hamada T

更新日期：1996-04-01 00:00:00

abstract：:Injury to vessel walls, especially microvascular damage due to free radicals, has been a focus of interest concerning the pathogenesis of systemic sclerosis. Excess reactive oxygen species may induce antioxidant defenses. We therefore measured plasma superoxide dismutase (SOD) activity in patients with systemic sclero...

journal_title：Journal of dermatological science

authors： Morita A,Minami H,Sakakibara N,Sato K,Tsuji T

更新日期：1996-03-01 00:00:00

abstract：:Bacterial superantigen such as staphylococcal enterotoxin B (SEB) induced strong ICAM-1 expression in organ-cultured human keratinocytes. Other superantigens (SEA, SEC1, SEC2) but not mite antigen (Dermatophagoides) also induced ICAM-1 expression both at protein and mRNA level. In contrast to ICAM-1, vascular endothel...

journal_title：Journal of dermatological science

authors： Matsunaga T,Katayama I,Yokozeki H,Nishioka K

更新日期：1996-02-01 00:00:00

abstract：:Etiology of connective tissue disease is unknown. The association of infectious agents has been suspected serologically, ultrastructurally and recently by means of molecular biological techniques. We extracted RNA from lesions of 25 discoid lupus erythematosus, 9 systemic LE, and 3 systemic sclerosis biopsies, and rev...

journal_title：Journal of dermatological science

authors： Fujiwara H,Fujiwara K,Yotsumoto S,Hashimoto K

更新日期：1996-01-01 00:00:00

abstract：:Transdermal system(s) bearing captopril were developed using a low temperature casting method and aqueous based polymers viz., eudragit RL-100 and polyvinyl pyrrolidone (PVP). The developed system(s) were subjected to an in vitro characterization study. The results were compared with the transdermal systems of the sam...

journal_title：Journal of dermatological science

authors： Dubey BK,Katare OP,Singh R,Jain SK

更新日期：1995-11-01 00:00:00

abstract：:A simple method is described for the procurement of human blood eosinophil phenotypes by combining an anti-CD16 monoclonal antibody, immunobeads, and a non-toxic and non-ionic density gradient medium, Nycodenz. The purification depends on the removal of mononuclear cells using a 1.076/1.102 g/ml Nycodenz density gradi...

journal_title：Journal of dermatological science

authors： Miyasato M,Tsuda S,Kitamura N,Shirouzu K,Nakama T,Sasai Y

更新日期：1995-09-01 00:00:00

abstract：:Clinical analysis was performed on anti-cardiolipin x beta2 glycoprotein 1 (ACL x beta2 GP1) antibody positive patients with collagen vascular diseases. Nine patients out of 89 showed positive aCL x beta2 GP1 antibody which was a relatively lower percentage compared to that of othoffanti-phospholipid antibodies, such ...

journal_title：Journal of dermatological science

authors： Katayama I,Nishioka K,Otoyama K

更新日期：1995-05-01 00:00:00

abstract：:Surgically resected specimens of skin appendage tumors, spiradenoma, poroma of eccrine glands, syringoadenoma, syringoadenoma papilliferum and mixec tumor of skin were evaluated for tenascin, as well as the basement membrane-associated protein laminin, and the extracellular matrix protein fibronectin, using immunohist...

journal_title：Journal of dermatological science

authors： Mori M,Shrestha P,Takagi H,Ogata K,Takai Y

更新日期：1995-03-01 00:00:00

abstract：:This study reports on the isolation and characterization of a cDNA clone, regulated by androgen transcriptionally, in male Golden hamsters' flank organs. Previous studies have reported on the cloning of an androgen-dependent gene, FAR-17a, from the same hamster organ. After castration, the FAR-17c transcription rate d...

journal_title：Journal of dermatological science

authors： Ideta R,Seki T,Adachi K

更新日期：1995-03-01 00:00:00

abstract：:Keratinocyte growth factor (KGF) is a stromally derived paracrine mitogen that belongs to the fibroblast growth factor (FGF) family. It is secreted by dermal fibroblasts and specifically promotes keratinocyte proliferation. We have recently shown that epidermal growth factor (EGF) and transforming growth factor beta (...

journal_title：Journal of dermatological science

authors： Latkowski JM,Freedberg IM,Blumenberg M

更新日期：1995-01-01 00:00:00

abstract：:Epidermal turnover time is the time taken for the epidermis to replace itself. The turnover time can be subdivided; when the epidermis is divided into proliferative compartment, differentiated compartment, and stratum corneum, the total turnover time is the sum of the turnover time of each compartment. Using Weinstein...

journal_title：Journal of dermatological science

authors： Iizuka H

更新日期：1994-12-01 00:00:00

abstract：:Survival and extracellular matrix gene expression were studied by viable cell count assay and Northern transfer analysis to compare the sensitivity of normal skin and keloid fibroblasts towards x-irradiation. As the dosage of radiation increased, the numbers of viable cells in irradiated groups were remarkably decreas...

journal_title：Journal of dermatological science

authors： Lee KS,Jung JB,Ro YJ,Ryoo YW,Kim OB,Song JY

更新日期：1994-08-01 00:00:00

abstract：:Cyclosporin A (CsA), a cyclic endecapeptide, is a T cell-specific immunosuppressant and is successfully used in the field of organ transplantation. Another T cell-specific immunosuppressant, FK506, a more recently discovered macrolide antibiotic, is effective against graft rejection at much lower doses than CsA. Altho...

journal_title：Journal of dermatological science

authors： Yamamoto S,Kato R

更新日期：1994-07-01 00:00:00

abstract：:Extracted human terminal and vellus hair follicles were prepared by EDTA and examined by light and electron microscopes. These samples were morphologically well preserved and enabled us to observe 3-dimensional (3-D) views of the outer surfaces of hair follicles. Interestingly, the bulge areas of terminal and vellus h...

journal_title：Journal of dermatological science

authors： Narisawa Y,Hashimoto K,Kohda H

更新日期：1994-07-01 00:00:00

abstract：:In order to investigate the mode of action of testosterone (T) on human hair follicles we studied the metabolism of T and localization of androgen receptors in outer root sheath cells (ORSC) and dermal papilla cells (DPC) from different body sites. T was principally metabolized to androstenedione (delta 4) even in bea...

journal_title：Journal of dermatological science

authors： Itami S,Sonoda T,Kurata S,Takayasu S

更新日期：1994-07-01 00:00:00

abstract：:The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation and collagen production of normal fibroblasts were studied using four WS fibroblast strains. The conditioned medium from the WS fibroblasts brought about activation of normal fibroblast proliferation, whereas, that from late pass...

journal_title：Journal of dermatological science

authors： Hatamochi A,Arakawa M,Takeda K,Ueki H

更新日期：1994-06-01 00:00:00

abstract：:In order to disclose the possible disturbance in regulation of p21ras function in neurofibromatosis type 1, we investigated the recovery from the quiescent to cycling states of the cell cycle in the skin fibroblasts of the patients. When cells cultured in serum-dependent medium are deprived of the serum, these go into...

journal_title：Journal of dermatological science

authors： Kitano Y,Saito K,Okamoto E,Hashizume K

更新日期：1994-04-01 00:00:00

abstract：:We have studied the distribution of SH groups and SS linkages in solar elastosis, in comparison with that in aged and juvenile sun-unexposed skin, using N-(7-dimethylamino-4-methyl-3-coumarinyl) maleimide (DACM) stain. In heavy solar elastosis we found increased fluorescence for SS bonds in the upper and middle reticu...

journal_title：Journal of dermatological science

authors： Pravatà G,Noto G,Aricò M

更新日期：1994-02-01 00:00:00

abstract：:Annexins (AX) or lipocortins are a family of calcium and phospholipid binding proteins that have been implicated to play a role in the regulation of inflammation and cellular differentiation. To investigate a potential role of AX in skin disorders we studied the distribution of six different AX in normal human skin (N...

journal_title：Journal of dermatological science

authors： Bastian BC,van der Piepen U,Römisch J,Pâques EP,Bröcker EB

更新日期：1993-12-01 00:00:00

abstract：:It has been reported that various anti-psoriatic agents augment the beta-adrenergic adenylate cyclase response that is defective in the psoriatic hyperproliferative epidermis. Recent evidence indicates that cyclosporin A (CsA) and FK506 show beneficial effects on psoriasis. Using fetal rat keratinizing epidermal cells...

journal_title：Journal of dermatological science

authors： Takahashi H,Iizuka H

更新日期：1993-12-01 00:00:00

abstract：:Isolated normal human melanocytes became enlarged and more dendritic in association with an increase in the activity of tyrosinase and the amount of b-locus protein when they were cultured with 0.1-10 microM histamine in vitro. However, histamine did not exert a proliferative effect on them. The stimulatory effect of ...

journal_title：Journal of dermatological science

authors： Tomita Y,Maeda K,Tagami H

更新日期：1993-10-01 00:00:00

abstract：:Using fetal rat keratinizing epidermal cells (FRSK cells) we investigated the effects of retinoids on the cyclic AMP system. The beta-adrenergic adenylate cyclase response was significantly decreased by the treatment of cells with 1 x 10(-6) M Ro 10-1670. The effect was observed by 12 h and remained for at least 48 h....

journal_title：Journal of dermatological science

authors： Takahashi H,Iizuka H

更新日期：1993-04-01 00:00:00

abstract：:We have developed a new cell culture system which is of benefit for the research of dermal-epidermal interaction. In this system, normal human keratinocytes are cultured on the upper surface of a permeable collagen membrane, on the undersurface of which human fibroblasts are simultaneously and separately cultured in a...

journal_title：Journal of dermatological science

authors： Mitsuhashi Y,Mikami Y,Mikami H,Ishikawa H,Tamai K,Hashimoto I

更新日期：1993-02-01 00:00:00

abstract：:Malignant blue nevus is uncommon compared to its benign counterpart and is regarded as a rare form of malignant melanoma. We report the clinical and histological findings in eight cases. Histologically, all eight specimens showed no epidermal involvement and had contained within or were adjacent to portions of blue ne...

journal_title：Journal of dermatological science

authors： Mehregan DA,Gibson LE,Mehregan AH

更新日期：1992-11-01 00:00:00

abstract：:The initial lymphatics of the human skin do not possess a basal lamina, but a subendothelial fibrous network, of which we do not know the biochemical and immunohistological composition. We investigated the wall of blood vessels and initial lymphatics in normal human skin for the presence of fibronectin by immunoelectr...

journal_title：Journal of dermatological science

authors： Lubach D,Nissen S,Marghescu S

更新日期：1992-09-01 00:00:00

abstract：:Rhenwald and Green's technique is currently the standard method for growing stratifying epidermal cell cultures. The serum free system developed in Ham's laboratory (MCDB 153) was designed to grow keratinocyte monolayers in clonogenic conditions. Our aim was to optimize conditions in serum-free MCDB 153 for culturing ...

journal_title：Journal of dermatological science

authors： Boisseau AM,Donatien P,Surlève-Bazeille JE,Amédée J,Harmand MF,Bézian JH,Maleville J,Taieb A

更新日期：1992-03-01 00:00:00

abstract：:The usefulness of the Yucatan miniature pig as a screen for skin dipigmenting activity by topical application was evaluated with standard compounds. This is a naturally occurring breed of swine with light brown to dark brown skin that is relatively hairless. The skin morphology, including the pattern of pigment distri...

journal_title：Journal of dermatological science

authors： Nair X,Tramposch KM

更新日期：1991-12-01 00:00:00

abstract：:The effect of DOPA and glutathione (GSH) on enzyme systems for 5-S-cysteinyl-DOPA (5SCD) genesis in murine melanoma cells cultured in tyrosine- and cystine-free medium were studied. DOPA at its optimum concentration (10(-5) M) when added alone did not alter tyrosinase, glutathione-S-transferase or gamma-glutamyl trans...

journal_title：Journal of dermatological science

authors： Chakraborty AK,Ichihashi M,Mishima Y

更新日期：1991-09-01 00:00:00

abstract：:Psoriatic skin lesions contain HLA-DR positive T lymphocytes, and other activation antigens, which suggest that the T cells may be producing lymphokines. Gamma interferon is produced by activated T cells, and its presence in psoriasis has been inferred by the lesional keratinocyte expression of 3 gamma interferon-indu...

journal_title：Journal of dermatological science

authors： Barker JN,Karabin GD,Stoof TJ,Sarma VJ,Dixit VM,Nickoloff BJ

更新日期：1991-03-01 00:00:00